	   SnapGene      ôGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAATTCgccaccatgcagatgtctccagccctcacctgcctagtcctgggcctggcccttgtctttggtgaagggtctgctgtgcaccatcccccatcctacgtggcccacctggcctcagacttcggggtgagggtgtttcagcaggtggcgcaggcctccaaggaccgcaacgtggttttctcaccctatggggtggcctcggtgttggccatgctccagctgacaacaggaggagaaacccagcagcagattcaagcagctatgggattcaagattgatgacaagggcatggcccccgccctccggcatctgtacaaggagctcatggggccatggaacaaggatgagatcagcaccacagacgcgatcttcgtccagcgggatctgaagctggtccagggcttcatgccccacttcttcaggctgttccggagcacggtcaagcaagtggacttttcagaggtggagagagccagattcatcatcaatgactgggtgaagacacacacaaaaggtatgatcagcaacttgcttgggaaaggagccgtggaccagctgacacggctggtgctggtgaatgccctctacttcaacggccagtggaagactcccttccccgactccagcacccaccgccgcctcttccacaaatcagacggcagcactgtctctgtgcccatgatggctcagaccaacaagttcaactatactgagttcaccacgcccgatggccattactacgacatcctggaactgccctaccacggggacaccctcagcatgttcattgctgccccttatgaaaaagaggtgcctctctctgccctcaccaacattctgagtgcccagctcatcagccactggaaaggcaacatgaccaggctgccccgcctcctggttctgcccaagttctccctggagactgaagtcgacctcaggaagcccctagagaacctgggaatgaccgacatgttcagacagtttcaggctgacttcacgagtctttcagaccaagagcctctccacgtcgcgcaggcgctgcagaaagtgaagatcgaggtgaacgagagtggcacggtggcctcctcatccacagctgtcatagtctcagcccgcatggcccccgaggagatcatcatggacagacccttcctctttgtggtccggcacaaccccacaggaacagtccttttcatgggccaagtgatggaacccCTCGAGTACCCATACGACGTACCAGATTACGCTTAGTCTAGAGGGCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGT  9é                   $         '            "      -   #               !                     "               '                                 $                        !   (            .      #   ,      #      3            %                              (   "                     2                                 $                                       $                                                         !   "      -            $                        +      
                                                                           *   %         "   $               %       $      #               "                                    
               $   .   #         1   !            #         %               )   "      $      
               #   !      *      (            /   +      !   "                   *   "   /         ñ yVß–/	0	1	6	;	J	K	l	š	£	¤	H
I
rÝœº'©Z–¨  yzœ¸úN ¯—UL	U	m	»ùþ�ú(ª§OîY&Ú – ò �ÞW†2	7	¥	Î	V
ˆ
u p»[ n© · `æÅµà'E‚<	w	›	J
›
;sÏÞ·k=ïS¯—. C “�ûO}€ÖGiµ. M	Ä
’ž¼$ª)PÛ$ •ç°ò†÷BV4QÞ	V	Œ	ì
d{Ãú°Fr¤ûµÚ`¨Ýk«ïˆ'7 f{¡îP	n	Ñãé ¬ÅY‹ž«�f:€›Z§�Õ z¹:Z½˜A	Ú
«ÿÎy…€B' �D÷’Ø(Xaf‡ÏÕÂ»Íâ	3	8	t	Ï	Ô	‰
v¾!—Cq¼1×€–4 $žåêsš¦	Ñ{�Ê :_u¼é
G — LmF×	W
�
¯
2{gÕ1˜\‘Ðoª F ó þß!N!·ffÝ\‡ãÀž" œ açî+£‰	½	Ë	tÐ^)v­°[.>t�ó4S°}l8N˜/ o ®õR©¶É*üÈ&=	d	l
“Û<±Rø,Ö- t 40\(>ËD	sx	€	œ	/
K
‡ÏJ•§)1Le¹ÂPatßúl‘¸l‡nð¤T‰L# ¸ +ìÆËþ1[g»áërÐ÷Fƒœ
*gm¡ÆMhÊÕç™`°» D ü~dÀ¹þ€	N	‡	ek�“Ÿç'¯,/s Q P�òjl/MÂÝ%?F*�y)·Ü M ”ž`àÄ[	Å
«Æ/‰äÙ÷o? X y×Hn¶�=}½œ Õ§UŸÊwÂo´}! ˆ ÛŠó5C²ßW	—ßˆôû±Ò¤¥a©‘®ÍC¼¬»ð±a 8ƒ±ºø¬	�	|ÄA�G!c{ü¶7Þ2p‰›8˜¾ ‹ –ØWWeŸ:³s£+(@�  è‡O¾5R‘	í
û£íºÛü±lq  ° G§­ïAoNo	Œ
äUT­Æ,ŒÿÃ ½ g¨
›¬LŽAJq[‚ Ï |‘¢©tpQ	]
ƒ
$B÷ZàHNg'���" €ôŸµ•Ò×ê!oÀÉ�Ÿó;FNœ¨fx¦¨¹ÝÖÞæ Q]†Ùâ“Ý'g¬
¾
¬ÌèÏ2�"aÌÒ } É v°�Æ~™_iB	Z	ßE†>?è2 Ö {rºC[‚ö1wŸÛ
N7 bXCD1 y 8CR;¾J;[‰Øæ	t
ŠŽ„"z¼&*I}{;' ‘dø�“ü)DYXg»ðÆÎ4	9	u	Ò	Õ	™
Ø
byÁ"ÀDft2®”—i¾2 EÙˆÖ°¼Lä	Š
Ž ™yÈÀþ
 < > »ñŽb 	Ð	ikw¿^rw¯Øô<5Â0 R çÐ9Ãâ£Íãc
˜âÈ½lö�g…|ÑÃ O ë	¸	–
‹ÇÚzê>ý å %mŸæÊ^±‘à!;`Ûù´ûÆ + `–½-›§	šâÃEÒ/?8|Ëv¶½ÑH2‘ 	 ³ ²Ë#VótÐ]j¦ @ Æ d­»Á
ëŽhßíæ*è‚ª¸A´= " ˜ Û Ø	3qÖ�™Ôzìq’ÄÑp Ÿ Mn ¤cGmˆ¾°
îxÂp2öV¿Cú$ û dBSb§%d(
X
�
Í
â
3`|Óò­jxÓá‹ ]�¿« G Ç8›½F÷gÞ9@Ï ô T‰Ü3–Oã"	9
m³ûi…î)g+ˆ7­ï!Q …ÿªÒà3�é{	¸;*]äÝv ‰À"":ñÇ
€È“•1ÁŸ Jkèr	Š	»	É	Ì	·÷\_w®š%/ryŽ~�6 V ® U�ž*±0u5qwm9™0 � Ä bïaâ	ÑŒ\Ý6ëôT¬Oø! T ¤ ,ž¤±	¾	Ç	Ý	÷	ÿ	muµÚäè˜â#?`n±Ã-¶d°( ¯ S	¤%.‹Ž˜+ ©>	a	ß	�”ØÜbo¨²ÜåÉ²SVl!$/¸¿×n Z p à?T¤ýF	ž·æÿ®WâÄÏùl™Æ‡  „ ö·Ê£ó		e	À	£§ëïóe—×êèÿ¨-ßåd   ªéùJ
.É'm
	=¢øÚÓ². J ¦ 5Yäs?ÃÌÒ®
t¦à’i	—	?KMfÃßQbm¡¹ˆ§m]oðÄìñÊi± e 9]›ê	*2ºu~’QÌƒ¥ó&# 8 u ‘ © )1o)«v�	
0
e
–¨¿k3Q©ÀÊmÌWïI†³õUM,  1 {mÙEéy	�	
>
L
ˆÐµPä6àûÚˆäAQb‚%dp¾åÅíd‚Š<W–D~  ¸,
!hnX[<´ê7Ni×W„¡èƒ¨±ë¼# ' ¹ rÆœhâpìsñG°´åó	#
�
Çæö>ËÖLYÐ¤ ôfÅ 0ñ»Ç2\Å‘Ñš¥Ï+qw¢/ïùZ¶¿™š-Ò3 ,<J¦ÏíýÕ±Ìÿ¼Â0Ü;kŒ±ÏäFïø„Â³	ù	
£
·
".8Cwš|
5´ë
aJ • xMÝ´&�I	¢	U
‡
tqýÜ¨~R®%-m§ 9|Õí´Aÿ3	@	O	‘âèªÄ„´ÙAe9š¦ E ý'e™Áºá	½f0ÉÜt% n s › í0=ûr¢c	ˆ	†’ÎÚfl” ¦(°ù -ŒÉÔæ R|+k ckm¿LŽÃdÜ&Ô¦T+.nˆãö Š ‚N«�	–Þ‡@¢¹­°1Bº°*p— ¼ ¦�¨ós´o GK&EŽ§@�þ¸Ý Q Œõ&I0hˆY	«
ÞD‰>z Q º�Wïá¯ÅKb
˜
×
�®Ç“hýf½Á/{Â  * N ä l•±Ê"U\	™áŠDµÐÚøü³ ! Ú ú ŸRa$l¬p2¬ÒŠÓå¾ë)ÿp·@¾ 2„ˆ¿7€è9zÆ
²Çúö*0Ü  j`º	Æ	á	ö	þ	l´öŒ�‹ç/—x«pµŽ÷~( Y $Š�—øIS£	-¢E	`	�¢¶×êþ¡Öáçþ§ÃÎUk #˜Ùmäc" d XnzršØÂèß–	=
>~´¾Þ¨ËPVlË�c½ëìòV• & °o·ðE�î™³³é µ	XÏ£§ m r Ü8³Ôì˜³úàH	üÿe‹ÈÓå8}™­j¥$, ‰ §‹ô¾�³X	�	ÛÿˆÓ¥¸J=¯âADý ¹ÉƒVQ#6®J~˜Æà‰C­)’½Ï¼û²¶zÁ % c Wm–RDÅ	<
¡é³õ‹².àý¦´bªêÎñ�b —²½ù­Ž	} Åè²Bdßü¬³Š·ÇÒäIá7˜ÎŒy#À2 \ ¯Â–»¼þz
{
|
678JK°±HÆÑâãßà3456q–—¶·‰Š‹xŠœ	 !"™š¿ ] 'è°Ãâ9!ÿ	C
9LI"Ûïø|±¸Ä9›£ ¡g„Œ}
ç
�#ý8ûÿrÝž‹�
Ÿ (ZŒßé]™ÄãÙX 	:f YJ´#Ò,Ã¹òn:Ž á Wu>û]	Ô
;Üäù“›,T^)AXœ¤ ^ € ë :—	MX\®öRð}ŽÙ²äã  Œ ç ±"D
Ht`¤ÅÉù @oµAè¿R ¯mcŒ j ÌyéNØÿ6î
ñ
GüA‘˜»b²ü  ¢Ïh¦Wˆ~
è
(¤î½$Ï#žsÞDÈržÙ !…P+S�’	ø
¹9þÜ9ý5 ŸÈ$ 
)�êB^šäTž¹ÝåBpõ
CO~	�
HN®'AKµÓdJÎÔî † [à®ð€�?¸Y	;åõ5¡Ç$a�Ä‹º×ov{ ± bÅ¨¥bg-9¾x€Ä;�  ÄûH¢k.Úg	p	œ¥äí`VUZ-¥óò 4  â ÿ jvhKæ^	©
Õ
‰åjþ»'-k¥ XüÐÝúª­GBœá\*� ¾ ÂÅ<œÓÕY‡ À ?[cgA›”M�KrU_BHƒ � Ì Ó NƒÖ�Úª«			R	/øžÆ~Ú:å#…^s Ð Þ ì ÷ ø/<;vqÂ	„
Y¯÷Öh(�³á[ _ ˜}^
%CNS\ËIñ¸‚‘f‰“¶ ˜’£uÛ%	*	]<‚-€[áO�Rä$ 65¶	E
 
ëI½”™_Ôìöu'I/§×$ è ¶¿Z²õy| #í–ØKÁÙ>�Aê)6a~¥“Æú•gÇ‚j  - � 'Ì·þ
©ñÓ",Ê 1Í¿<O�Ê	\©Þçß �îõ�Ù©	ÇGp�„œôG©#ÎØy÷ºç µ ï "œãw¶õLÉé(”Ëj
­
ÍÏ–¢XMW5Ó AA|}Àe­ÊÐD�úBnHd R^‡Ú+”úB™h&
”
¿
Ë
é¤x�Í Â pÍÞit¯	Û	Si3‚!°#Â+b€: zþjÝ17C	àB ?c†³é)  h k ~ Íw±Òê”O)[	ï
Hý?FÙíöŒ–ý¡ Ê ´%XÙ `’‡™Ô¼ti…´ Ž�’Çší	ò
”@¨3 £&-ƒ0]‰ô2 îé
8¥ñïc
èUO\ÜìÚ × H…XyOD$ŸªE |ª»i5\§ãxï	
¾EßÉsî  sÐD÷èÜ
ô
)¨%ÐY†t2ÊŸÍDJ z 9v<\ç	‹{½'TCÝŠt†<a® ?¬Ÿëû¿Kf‘ÕŠl“	º…ˆ+OïY ô 	·<Qo
�1x:Jÿ‘×£È D‹"SO†œ,T_ŽÙu
ù
#•¸:~þ6|ÉÕ! Ž_›åùÄUŸÞ…®TD.	5	:	T	k	v	™	š
:o¶j¹<îÿ" B ’e”æYh¼ñ…ö-PÝÃ
Ù
ë
czÂ�#¯ÍEXŠ©_j‡ Cr‘×E ÎÔ Ó	Ö	Ž
1BÔe^•»è	†â¿3FÏï- *3­ëôQ¨/[Êý*ZfºCqÇÏö	IÁÅO(Lgu�¬Zò3¯Q˜_KºÕ L ñ_ß		†	<|�æÅÉÁ.ŒØÿ(¶| ‡ Ú‰×¹4±½úž¢ó£bz�Ì»?`Œ  Î F¬ž@M”‚
‹
§
AÖöTSnÈšŽòMpwÜ$ x È Õ 7P\…Øáq’Üu¯�ÅZv×å	½
M6!» %W`ç0Ë ; = �æ8¢Ñ	ahjxÀáesvx¿óõ„	 ² ¼òÏ·	Â.>°ÆÙy=1Å� ? c£cA¦cº½á
_ñÁo€wç�Ã³< Æ©Ñ2âð	!	lh„’í(¼:”6¬îu S £ Iq	Ù[Üá5_qmÂ5 ƒ £è¨	�¦åîa­§VdÈ÷kÅ†ÞÒ  0 7 � ¨ (ãlªÑ­h	d
Oã52 É¦ï‚Éôh%C}+  ¥Åüº/Û:‹°ÄÎã¤¯ÁÎäò	 vW™;{	.6=¾ê‚,êóIÄ ” š wìL	¡	T
†
s¥ÿÈÛ{¦
 K~ª
S¬¦?õ–   P Ù ã ù k1‡¾çy«a
—
Ö
±ùŒ(þ.  i__	¹	ý	ŠæOk¼jë”l $ b lÄ	<|úµü+ & çf‹ýB
5Þû«²áH°µÃÍÜ�wž à æ Y=úÓ
[QÑ_ÂØ’øã
â] nËFQ'Î®"a�CÇ 	aÃúS�œ·}	›ã_4`wÖIÓ3í ¿ JZÄÏ@FÒÔ Ò Ý —Õ.;)	.[�,
�Å9·àZ]rˆ’ , ¾4¨	ý
Æ¼F=€Óõ05ƒ}›Ì&H¾’”ÍÆi ´ Á î !›Ì*ô@ù{dsÚ	i
hÎœ•¡*WGLV 
 g ³$WÑì	Ø…§(s„ ,â\x§ëíCI >u�^kw”BN‰�ÖîXs`­ A ¬P§qÖe­S	Â
0œ�«iY;ºç…áE¹† w Ç Í Ô O„×p‘Û«Ö�
¼
ùmÿ-�ñLµ>_‹ : ¢Ð¼	à
Áƒì»Àæƒ«t ‚ ¢ ©¬®À
	ñ	¬Ø45ŸÇÛà4î+é$BH “ ™ ø 0xS
`
…
r°øi“• # a ß <ekŠÃ	Ò
4EPZ×{¯´Â÷â	v Ñ Ü ù3¶Z^ÄÎ4‚šG‘“ÌÅÒ\hq í  š=+wrÙ	h
Í×ˆÕ)íWrê¬ì ¡ Oo¡¦Õ¿»
›«‚3¹K„DŠ ` ™d‰R
_
g
Ñ
DOY]ÌÃ®Á’Ëuëg”*   N¥H^>±
²
TUVWX˜™šªÌ€�Sy÷øRƒ­ÀêRS% ~~½ I_n„?õ



2
³
&ÍïTJzòqñùWœµSðûT· « ™

)
´
'Îð.�óâKo±k¤ÔâÚ›“À ü !“¤+:`våTc¨eoz…@‘
\ó  OSáñU" J7Ü&¨&	+	ƒ	

Î
	^=}Uòü
]®�¶ŒTå%�ü$ e¾CýÒö

3
Y
ã
4ƒaÔÂP{r�òEKúXy^N¬¸ }_F
(Ï/Jªpødl¥ÕÛœ”Ø 69·Y�r
æ‚š¾`LG ÁÐ Èì¾Ævhjß(:0>A¨Ï H ¬ 7šœ	
!
*
¡
µ
ñ•âôÕãí÷²O[ãJ%  "ö;a¡$G î?fp†ì—n´üjÂÚ‚¦,Ðfû–T®âk  é UŠ·Ý[—³”¥P}æU:
ö
¬ôÙ†ý*ˆ'?”ÇhR£V$ õ ý À4Û,wz©ë×äA#	*L?]£'7bh¹‰!PÌð"€ƒFy uFµMdÓ{’
ï/BëôŠÒæS„Àv¡„8Èò#   . Ž áÍ6ÌÝ¸	,	„	_tzÔRËV<ÀÇ^å]&ý ( à|©'	{	Ÿ	@
ó^MUËÞ Ó8‚
Ï
Þ

~¹ý¯2C+�ïPžèF‘ªßèL  †«K4ÈÊ'”ê
ÿ
ªò>#-¡rž·Î=
æ¡wà ÒÆD#Z
+5@b”q|…Fz–õÏY£ø­" €€ˆ¿‚Š­5o#–ÚÈ
ä
„ÈHQ” ‘ó�ûç2$ŒÕÙ» Áö‚Ô;Óò÷

Õ%8Ã‘sLtHªÂ˜ O 3 fïùþ)Çêª	
4
N
�ŠÉÒ	·ÜYf 4_z¹âè ¶ ð x·•Í	G
ºÎø~›ù&¦YNíX•Ù ~F‹	Ð«xq£ŒÜª6€Ô #�äKléö`MÌs	®
z–¶Ð0ez�—Ö9[�� ÈÊê)%¼	Ê	.
k
)0Kd¸]`sk¯†ms£÷ˆ7M W x~¸ÁýZj®>›ŸEžvrx³n> 7VŽÑì9² 6ûoš½ ¯ n\
#¿+ßMIe¥Âå | BB}:^fžs
�Ëçƒa)1H!=|C:1Ñ cðûCºÌã	]¿˜Çk­;1Ð
 _,Éi•
¥]7ßú ž Å b‡'
À
Ì
íÒê�wÞiìõUàyB©Žù SˆÛ•š8
E)8?PÎ U ­ Ã �°	È	Ü	T¶ã™"$ê,vc¯  ß->Ÿò	¿	Þ	ònÛ–äé±.·¾±$ I ¥ 8¥u‘é	

jP}¿Ù‡ãî@HP\a$o…²ÃäÄc�;°. /;IqÎðÔ›Áj²	ø	 
"
+
¢
¶
!-7BpZvåõÖîø4VYƒ³˜KÑe# &/{%<@q¡2?	b	…‘¼ÍÙáp©ÃÛøƒ§³Ø@Qd*l » 
¥�ÿbr%Hg‡ªn•Ýc†C¡-m‡%0Ž¹¯o  ) ° !TŒîk8o1õÑ´ÒgêüeÛÀ½1 ¯#q‰Œ™÷¢Á,¡çïÞí˜²D	•	à	õ	ŽµÖýk³Ýèæ–Ê
€ÍTW"—¢¦UØãö! l q ¸È‚V7•¦²ÓëQUP"ŒIG	;
Úþ‡Šþ-(é@i¤ [ ®Áá• y
æ
IŸç¯Úî÷"‰ÅÐúþ•ˆ¢   ê Vt‹Þ\˜~\	­õGXã+�ÈñmšSW i ?´Î@x¥çMV×þ*ð
÷
¸ @8¼—4ÇbØ# … A¡j¸Üä->¤aô	Bf	¤ìGôM&@¤8c½ŠuÍñz  þ iÁåbfˆiš©ýº&àjG† Ë ö M‚÷�ÙuªÚ	$	Á	;Ê Õ"Q#„eµ% µ&5YíôËx{€ìØ¶	Ÿ
¨ð+J“˜Ø^ë@é("[×.ö�æ vKÈh“|˜Ê®	%
“
Ê

É£CÁùAw94 ý�‘“Ü(0>ìõ‹“•ÓŸh³  ©%G„4/óçíî	ó
ð	çT…N[©ÉÛÌ Š«ž¶êNúe›Ôn
0‡S·¢…ÔóÇ) 2ó.ZÃÉ„ÍÓ„õ,SÜ-	j	˜	ê
9ÄÌWn‰¨¸ñ^íP]^þ"  K 6tÄ^Þ3u“	…	¦
@L{ÕS�òR¢ºVË_æ/'ÛÊ åÎ@17á¡¹ï¶	`g‘à'ðd=nuv“Åòí²$  / 6 � § ¤'¹âŠ¯Í§uN¦:cz1<é^p�ÁÈjÄÝ Ø ^†½J}ª 	ü	¤NR«¥ô
 ùA
&PÁÍœÌ Ô:IRœY(	|	ü
-€+3»	ô_E%ßì f )øáz�ë	v“„ ¦VF' 9 v «pÏ¨¬Õð	€
ß
À×3lº4ªþ¿X,ŽðàG ’ *2jµwÍ3‡FöVé«# }¼Ô]ƒ=ô	


1
Q
f
º
Ð
—©RËð›´JQ¬ÊtéïQ! ª 	*69äüy§Ñ‚	

Áñû	\n°DJ£ÙŸNš’àM |^Xœ 
q
 å©áÅNZci=Î*  tE´
LÚFŸÒê>Öëõ
«ó.ü¢&‰ÑåRƒ‡&¸¿Ï>euƒË¢Ex   ß5ÇÉË“z	ž	?
Ý
syQLBqÆîçE K( 2 ‡Ñ¬4nÅø(•ÓÆé
M
‰Ñ*?¶$7Û“esŸæ3‹ÔX—¢á ÇE$-
¹cy•rµj}…¢‹8ö‡©ìZL { wm]ü[
"iŒ=ë8Þ(<{²¤=¼ä ¹†Ë7
\—DÞ7ù/  .:HïÓ=Àñ�è	
 ,6AoYO|¾ØUX†íGé„—uÐ½b¯®+ ( º ÿ‹®pˆ$qÀíæm±”	ô	„�»ÌÕ0…ç÷•ô	Ì×PÑd�¡¥Ú¼õ >sÇ�­ÀU6LÖ!‹Hx
å
‰,7»!è3ða×! Lh�@ŽØ ìóiã=taµž
:FÉI~%?ü%ZÖ!~õ" ¨‰$ƒ�ü3g’ò—Û/æì­	$
É
È†R¶’žMZ~gËÆ  5 1ò¸ÂÈƒ+RÛµ	¥
�œ&9y0;t· ]u€’.ÃÉ Ó¼÷QŽIøû	û
,%£À›¤ë 
Îû´<¦ÐÔó


P
¹
Ö2ðú[š¢ØI«™P ³	3]ÄÞžÆÑ=’ÒÕW›Åp
rxÄ’KMhÓ¡ -Gî¿ lðû…�Ê
6

¸x–Ýì;76K»ã* =Kg§þ�×#Tò ‡2<å .`ë¬	w
ƒŒËÔ/9E}$6µºc}`}ÊÖ" 4 Ò²ÍPÃÝú��³Ð*HVÄÚ´	ú	
O
¤
¸
ú
$›\g¡ê/ EFÐÑŒÖðñ½¾1ÿ²Gùú-UÃÈÉ«	5
v
‚‹ÊÓ#D¹	
Z[ÿ 5b{|ºé  .4  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                          *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           	            	                	                                                   	                                                                                                                                                                                   $                                                                                                                                                                    	                     	               
            ,            	            	                                                      	               
                        /         "   !      "   #      #   †   Z                     W                                                                                                                                                                     *                                                                                                                                                                                                                                                                                           	    	      	                   	                                                                 $                                                          	       	     
    ,    	    	                  	     
        /   " !  " #  # † Z       W   q�  r£  rÁ  r÷  wO  |  ‚3¾
Š"H“
¼Â=ÀE�Ž<H´	ú
A\|•ÌÞ„›1”ÜÄ
) ï¡WèW‰:É,¦íÿï¨ÈÂ1kääÄ
) žæ� ,¦ÆíJbÿ%ÙÃ
 ïi	�	¾	À	ê	
#
žæhnfºÑ\b×û*5¶â6yë$¨‹\ô²t!1{yt	!?1t!1!y!ÒA¡&.Í4„Ì×lÅ
·þ!!ÂÈÅ!×ÀÉÖïâcˆWÎ¶%=žž‘ AÒ¤xº�+Ò}ÏÔ£²CœÝ‚|‚Î0 Q†Ù“Ì8?FÏDÙ·x:%s©sÂâl
t—¼
Ø†w}ŒfêqÅLgº] –Lï3ò¢Ë9àQ†Ù“t2�NÌjÂ `¼× …˜yç×Hâ ö|ÑñçöÑñûX¸‚Þ ;YÖá£]ç9â‚;˜öÑñfqÅy×‚w	›	J
Þ|CÅiÎtŠóW	e	£ë§	E��ž”Öƒ8þ/cI§¶	¦Å„'£ö	þ	t>°Ý©s»ßµÂ÷Q‚âK
W
l
‡Ït—¼Ú`‰\ª,¨¬n“‹,ü&>¢¥Py*ù“
£@Â6]ã$¹’*RüÚƒËFi£¸%·\ØD”ŠYWlª",
´i„†~þ�Ó‡åŒÔi	—	mìe¥:@ãF%¬ yç×H9â‚w	›	J
;˜â Þö|ÑñÏ‰y`¼?@6ãF£%BKÒ¤V ,íY]. ï°a	e	ó	
,
�£Øë	Ãžàæ/3i„ëuËð¤@ã¹’F%£`e	£ë¼*Ñ ž:E›§§¸Ý® *5	Ÿ
“ë¿%	çÛ¸d‰®Áu‰	vÒ+5@–Ïy×,3ò¢¨Ë¬n9“‹à#‰ŒŽÖT"âÈ–¥íÁÆ 8FÏmÆ ŸñçX»b8¼�
Ç
Ø
?FöÛÑÏñ¬ ¥D?{|„Ù†ÂËV‚ÜýBø¥+�ï?‰ŒyØâ Â z2@ Á
,
i„qÁÞ�•	Ý
¯h<±X§¦qÁÞ•	Ý
8FÏjXü&,>¢Xµ¾y [Š&;ÔdœŽ…oÿ ±"UÏ[Ö	1�ÔÏ|Ñé ž:›§Õ€o�óF¨x¹æ1òÂÈ$ƒ+RÛ„»Ì÷·×P]…ª3évP/Ýy¦ ‹˜+®
¦ß	M²ÜbåÉ]oSV!i×· +ìÅËþ:»à!½÷!E‚ƒ›
y`¯Àž¸¹x}7ìKDØ‡Õ»‰
µMdÓ/¡„ò:!½!yÀž‹˜+ß	²ÜåÉSV!×¦ ®
¦Mb]oiìóµž
I?ZÖõAoNŒ
­Éé(j
¢¸[ ¦ ‹˜+®
¦»	É	ß	nMX²·Ü\_bN®×åWÉ]orèƒSV!i¼×61òÂÈ$ƒ+RÛ„»Ì¸¹x÷}·×P]7ìKÊ	§ï—×êè-å Go	UT,ÿ-ƒôé
8O\( ÿ‹í0ôÑdÚ¼în	ã¬Å‹íô€Ø"×ö 
FŸ>ëÏeK ^Þ	…	{'y¸—þ€È"œãõL0jË7 î­
ïcÏC–Ý³éì K ’ 
F^ŸÞ>ëw	…	{Ïe'ŒîgüeÀ%/óíð	çTN[ÛÝ2�"aO-Gî¿ð�

ìÌ	÷š%‹ rC‘¢töÅ
Û
ZàNsX£/1šfËç1E!8?ÎÑ }a$GÝf†	„	Z
zÏÔRªp,zÛ^å£®&û¦Ð
ðú[¢Ø™Ûßû¬mÊØ	ä	î Ö!`Ôì7q
Æˆ Šó²W	Ò¤®C8
¯Cž‘ßLÅ
/rCöÛ
X1ä	î 7
‹ ‘¢tZàNs£ »	É	nX·\_N®×Wrèƒ¼6%Œî/óíð	çTN[güeÀÛ† ¸¡aºü +v¨@\ O5ÓøU ­ Ã �°	È	Ü	T¶ã™"$ê,vc¯ .:HïÓ=Àñ�è	
 ,6AoYO|¾ØUX†íGé„—uÐ½b¯®Jkèr	Š	»	É	Ì	·÷\_w®š%/ryŽ~�6	 ³ ²Ë#VótÐ]j¦d XnzršØÂèß–	=
>~´¾Þ¨ËPVlË�c½ëìòV•T ¤ ,ž¤±	¾	Ç	Ý	÷	ÿ	muµÚäè˜â#?`n±Ã-¶d°Ø ^†½J}ª 	ü	¤NR«¥ô€ôŸµ•Ò×ê!oÀÉ�Ÿó;FNœ¨fx¦¨¹ÝÖÞæ¸ +ìÆËþ1[g»áërÐ÷Fƒœ
*gm¡ÆMhÊÕç™`°»ÇE$-
¹cy•rµj}…¢‹8ö‡©ìZL… A¡j¸Üä->¤aô	Bf	¤ìGôM&@¤8c½ŠuÍñz& d t ¸ +4ì0X\nz°ÆËþ1[grš»Øá(>o·ÂËëDrèð	Es�Ðßî÷Fƒ™³x	€	–	œ	/
=
K
œ
*>‡ÏJgm~•¡§´¾)1Le¹ÂÆÞPat³ßéúMhl‘¨¸l‡ËPVl µËn�ÊÕçc½ëð	X™¤ÏìT`‰£§°òLV•» … ¿ þ 	aiÁÃúAJ¡Sj�¸Üä-œ>Z¤·aåô	Bbf	}	›¤ãìG_Äô4Mf&@ˆ`¤Ï8@ciš©½ýFºwŠÒÔÖ&IuÍÓàñ3jGz†íR^‡Ú+”úB™h&
”
¿
Ë
é¤x�Íƒ £è¨	�¦åîa­§VdÈ÷kÅ†ÞÒò^ € ë :—	MX\®öRð}ŽÙ²äã³	3]ÄÞžÆÑ=’ÒÕW›Åp
rxÄ’KMhÓ¡D ü~dÀ¹þ€	N	‡	ek�“Ÿç'¯,/s Q# a ß <ekŠÃ	Ò
4EPZ×{¯´Â÷â	vE d R^‡Ú	+”ýXnz·ú'BršØe™ÂÁè<Q™ºßhá	–	&
=
o
”
¿
Ë
>½~´¾�Þé1x¤¨:fËJPVlËÿ�0c‘½ÉÜët×ìx£òV•�ÈÍ69·Y�r
æ‚š¾`LG ÁÐ  *ô     Û          7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL©^”è'ÄìÞKÒ �k=ö4bïOíøù/€ø?¢êP.8�öÿÛç ýø>Ñë „«M3CïP|× ªíþ`>Â(=Žôá×AWôÓFµ4æ­F†@Ø?óûÃn'PNØØPaM÷G1ºËÓ¸S‚ô¶?ÞºÐâ¼pµ5 z­�=Ûmaà!GwuP˜m5èèA<�ôáÜ8#‚?¹ë\= Qïëü{Ó|óî½Õ´pÓx»ˆý†ÑõÔ­yž·Üü`öžqÛà^?Ÿp´Ø­£�úaU�»¸þß]®?NÛOxvC�óï‚¸ýJïàG…,~ÐSþD_€X|v+GJ´8zÜK«GÔO»Ü³43�!FÄ!qÂ¾ÛÝ8¶K´üØ?ñm¶Gúû¨‚ðÝÝê4œ§A:¢#à‘^jâ½-Ú=·º'��|Á8!ÐõCÒßêÛ´KÞ Þïô� Ž4 Ñ!bÿÛb×»àø=»ûýÒ#`)ªŽÄŸÙÇ'Ñ§:K‘ò¥8m>Çˆúä£ÓÛŠ*?ïü�¯¡þ÷¿x¾¯–U„£lÚ.B-®k€ €45 hdÐØà0€î`ë„0T_N î@	¿ÜÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Å¥® Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û�¸NkÑI!:ª>Cª5TïÀN‰›Ú¬†ÔGA„*Í†…Ô`hÜÐ®ðó  D9¬9‡€Ýtu°ã¸cÏ"¼8´Í ÏÂº¨µ‹Â?……ãAÄ4Ì9²qÀÊú3N#±Ê-áLàãš� Ðnø¶íú;ãÄT›Û`á�c8ÙÎ†ðçãö±÷ÔÅvÍ±17'°`SÂè±´nM´³=<^+=Ô36?1ì²Ø1�Ž�m‚X<ê¸Û ðà"9´“(9ü†háDè0Å€áó¯²:œ&Ï;ñ¬Ó6Ï®lúèq+®î ¬VSý~=·«÷Ï>¹…B€ë²«ÀsÃ�†ûÓ0íŽ®³÷ýz*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹‰Ÿ,~ääNƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË¿þ`  œ  	e  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ¿<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4750-4766" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4726-4742" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4819-4840" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-10" range="4774-4780" color="#ffffff" type="standard"/><Segment range="4781-4798" color="#ffffff" type="standard"/><Segment name="-35" range="4799-4804" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6745-6849" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4556-4677" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3507" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2480-2704" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3192-3521" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2750-3178" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5128-5713" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="3" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3588-4382" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6675" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5884-6675" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6676-6744" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2442" maxFusedRunOn="2442" detectionMode="exactProteinMatch"><Segment range="2413-2439" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="SERPINE1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2442" maxFusedRunOn="2442"><Segment range="931-2406" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  m<Notes>
<UUID>5a372ea0-cae0-47ad-9e6c-c88d29e8cfd3</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCDNA3.1(+)</CustomMapLabel>
<Created UTC="16:0:0">2016.9.17</Created>
<LastModified UTC="9:15:35">2023.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="923..929" colors="green"/><ColorRange range="2102..2405" colors="light blue"/></TopStrand><BottomStrand><ColorRange range="923..929" colors="green"/><ColorRange range="2102..2405" colors="light blue"/></BottomStrand></StrandColors>  ,º     g  R         R7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL©^”è'ÄìÞKÒ �k�½7H0ó±'öqÉôiÎ’ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  ]      H®ZTR

BASE      2è  xÁA  ƒ°YëñÀ þµ,9,UYMæ$"¥�æÜ€‘ÔH©…Ûjk8b´¦Î›¤4±ÚFmRÚÀIè‚Ñf¦éV$s-˜Œ™¢ álÀÌÕ�šÑœ
Xm$Â‘	E³„	‹,+À„‚r@ºiÂTÐÖ¶åt[ÉBVFÎ¦+Z‘ª¦š*Ó1ÙT�®ÔÍ±5™²$Õ%šJ´”"§è67˜¤J4U[ C²‰"«MDQ3MkˆLÌ�4F	�&THE!A© ŒM¢m¡*ÕÔWÍ¤Ê À¶m”«©¹´6Sa!�k­
�Êaé†Eµ5ÓeÁ6Ò��Ð"4ZQåªµ©«\…B+­mÛr’«6¶mÕ†¸ÕVdº¥fÌšªºÍàh•¶  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  ]     H®ZTR

BASE      2è  xÁA  ƒ°YëñÀ þµ,9V:ÙšÌIDJÍ¹#©‘R·ÕÖpÄhM�6Iibµ�Ú¤´�“Ð£ÌLÓ­HæZ03EAÂÙ€56˜«!5£9°ÚH.&„#Šf	YV€	å€tÓ„02¨ ­mËé¶’…¬ŒœM3V´"UM5U¦c²©:]©›ck2eIªK4•h)ENÑmn0I•hª¶ †dEV›ˆ¢fšÖ™˜iŒ&M$¨�Š
*B‚R›DÛBUª©®šI5”A8€mÛ(WSsi1l¦ÂB ×Z•ÃÒ‹jk¦#Ê‚m¤#6 Eh´¢ÊUkSW%¸
…VZÛ¶å$Wml[µ!nµ"Ó-3cÖÔL·ÀúŒÂ  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>   Å<AlignableSequences><Sequence name="34_CMV-F_TSS20210702-027-00554_H03" ID="0" sortOrder="0"/><Sequence name="35_CMV-F_TSS20210702-027-00554_A04" ID="1" use="0" sortOrder="1"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  „<?xml version="1.0"?><Features recycledIDs="17" nextValidID="18"><Feature recentID="16" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="987" maxFusedRunOn="987" detectionMode="exactProteinMatch"><Segment range="958-984" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5220" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4429-5220" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5221-5289" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="14" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2133-2927" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3673-4258" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1295-1723" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1737-2066" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1025-1249" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1917-2052" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3101-3222" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5290-5394" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-10" range="3319-3325" color="#ffffff" type="standard"/><Segment range="3326-3343" color="#ffffff" type="standard"/><Segment name="-35" range="3344-3349" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3364-3385" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3271-3287" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3295-3311" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  l<Notes>
<UUID>5a372ea0-cae0-47ad-9e6c-c88d29e8cfd3</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCDNA3.1(+)</CustomMapLabel>
<Created UTC="16:0:0">2016.9.17</Created>
<LastModified UTC="3:57:5">2023.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  'C     g  R         R7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL©^”è'ÄìÞKÒ �k�½7H0ó±'öqÉôiÎ–ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  s<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="987" maxFusedRunOn="987" detectionMode="exactProteinMatch"><Segment range="958-984" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5220" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4429-5220" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5221-5289" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2133-2927" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3673-4258" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1295-1723" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1737-2066" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1025-1249" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1917-2052" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3101-3222" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5290-5394" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-10" range="3319-3325" color="#ffffff" type="standard"/><Segment range="3326-3343" color="#ffffff" type="standard"/><Segment name="-35" range="3344-3349" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3364-3385" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3271-3287" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3295-3311" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ”<Notes>
<UUID>555e470a-7448-4e3d-abff-425be138a8c7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCDNA3.1(+)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.9.17</Created>
<LastModified UTC="2:6:4">2021.6.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  'D     g  R         R7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL©^”è'ÄìÞKÒ �k�½7H0ó±'öqÉôiÎ†ä@ü©_ÎO±â>¹(ôöâŠ�ûÿ#ëè�B=ïÞ/«å•ahÛ6‹„�‹kšà € M@GY468Œ ;˜:áÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  s<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="987" maxFusedRunOn="987" detectionMode="exactProteinMatch"><Segment range="958-984" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5220" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4429-5220" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5221-5289" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2133-2927" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3673-4258" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1295-1723" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1737-2066" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1025-1249" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1917-2052" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3101-3222" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5290-5394" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-10" range="3319-3325" color="#ffffff" type="standard"/><Segment range="3326-3343" color="#ffffff" type="standard"/><Segment name="-35" range="3344-3349" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3364-3385" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3271-3287" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3295-3311" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  •<Notes>
<UUID>555e470a-7448-4e3d-abff-425be138a8c7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCDNA3.1(+)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.9.17</Created>
<LastModified UTC="2:5:59">2021.6.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  'E     f  O         O7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL©^”è'ÄìÞKÒ �k�½7H0ó±'öqÉôiÎ¹?*Wó†Óìx�®J==¸¢£þÿÈúúàP�{÷‹êùeXAZ6Í¢á$"Úæ¸  AÓP€QÖMŽ cæ¸CEôàä ›ýÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZZâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ¸û„æ½’Cª Cä:£UNüè™½ªÁ¸mDtA²¬Øh]F�Í
ï:CšÃ˜x×G[;†<ò+Ã‹LÐü+ª‹Qñø¼#øX^4CLÃ› à÷¯£4â;¢ÞÀP9 1Hðï‹nß£¾<EI½¶Ö0Ã�œèo~?k}LWlÛr{<.‹A†àqDÛ@;3ÓÅâ³ÐíC3cñÃnË!ýƒøèñfØ UƒÎ«�­±â Áó›AÙ2ƒŸÈfŽNƒX:û#©Âló¿Í3lúæÏ®‡ºá0à
Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~±Á°»ñ¿æ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  u<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1002" maxFusedRunOn="1002" detectionMode="exactProteinMatch"><Segment range="958-984" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5217" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4426-5217" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5218-5286" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2130-2924" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3670-4255" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1292-1720" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1734-2063" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1022-1246" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1914-2049" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3098-3219" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5287-5391" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-10" range="3316-3322" color="#ffffff" type="standard"/><Segment range="3323-3340" color="#ffffff" type="standard"/><Segment name="-35" range="3341-3346" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3361-3382" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3268-3284" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3292-3308" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  •<Notes>
<UUID>555e470a-7448-4e3d-abff-425be138a8c7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCDNA3.1(+)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.9.17</Created>
<LastModified UTC="2:5:54">2021.6.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  %@      _  4         47»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL©^”è'ÄìÞKÒ �k�½7H0ó±.DÊ•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹ƒ®ÁQ}8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸±X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Âª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡,plnüoù‚  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  w<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="15" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5190" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4399-5190" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5191-5259" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2103-2897" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3643-4228" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1265-1693" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1707-2036" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="995-1219" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1887-2022" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3071-3192" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5260-5364" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-10" range="3289-3295" color="#ffffff" type="standard"/><Segment range="3296-3313" color="#ffffff" type="standard"/><Segment name="-35" range="3314-3319" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3334-3355" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3241-3257" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3265-3281" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  •<Notes>
<UUID>555e470a-7448-4e3d-abff-425be138a8c7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCDNA3.1(+)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.9.17</Created>
<LastModified UTC="16:0:0">2016.9.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  „ý7zXZ  æÖ´F !   t/å£àµ£D] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÉ;_x•²Ï”²ö€ûjum{9:;(ïC…~f,mm2‰ç4‡¢Ÿw­f�RJz\ õ¿Å¤°\bI�ËýqC˜ÆTYíä`÷~åÀ‚û�ªõê·äM ™}ö‰/4ˆ€”uÌ´)ÕY¸0}M’fñDý� ê<‘od•&f9MÊ¸º�])e‚×@+Ì*¦÷?è&¿rr?¾(§3ÙÈž_.ìZ?\þIH_ªpÒ]Ñþï(·ÐqìCO„¼®ãïÛÒwú:;âZ|˜s!éÍ¹X˜
O}ÃÑ²Š+/â¹sŠ¶l%›œ8w�RêÏlBF¸ÁHVÞ`¡âšåcðw8@Ö§9À9ìT/vâ&}¾}OF–¡ûˆ‘˜§ÇÜñBÛ¡ä½PÔŠR‚[‚Ððú Qú�_:ÿÁõj‹6£–¯¡¶®¤Lˆ•@0$lÊ’+Ô})?
aÃc6A }.Åk*SØ3ðÈ
7�Çêó~nöíšAœÉCB¯ot‘V÷Ð
 ·OjÀ{ù~8Þ,‡8^�6†!æ�5?ù7ELð•5io kønÍŸp/—*xóáCëÖÃ†óÀ¢qã@ƒªQÎÉò�úHB¸�OYïm%L›–»®H –«û¨S&‘”löÓ£$?y{x¿QcDOb¾àåë°³¥‹ûìÈÄ”­í
ìÆÍõs”iQ•>æL
ØèP?‚çã±Îú½ëõOÀXáÓ WKoøè˜˜èïÛ5C�:šG×rDZDî*Ñ•c�c·J~Y(µNË×ÿ9ôp÷¶ng31üèB8ŠÍé<±üM“w­õ´S²g%fiôkí,\H�:?Ç±¦·‚Ó�ñ¹ñ`«Cy(BÅÖ¶Þó�™Kð@¬P¶j‘€r9&+V�*“SŠf'Ð–Ì{¡gê3†‚8]Âeöh)²tBÎÇ8·ï»L°}%ÂÔÄ¯C|[c%DR_áêw
Ó	&ÆMø³tÖ¬KÅÐÞ2ïùpáé��¦äýêÅJJª …‘÷cŽ• ¶¼¿áh8ùš±<ÆRª<%¢·�JùŒ5ÂÈ#í™#¢a¸šé«umÄ¿ÍõM|ÆIÜØ}…;³k1(,ù�”	ünÅÃ—Ãès[®£_wäüU!÷&øf[rh×Ž˜B×ÿ8F AñÉéW•.÷Ô®8xW)h�ND)ãÿÌrEàåÓnIŒ§6½ÚÚŒ²ö%O=^›Bai¯hž&û‘Ç³æzn•½¹ÁËi¡G+r®�›hëÆ–‡“óäà Òt^c¯NË:“÷[ŒÛD(J=Em#„=~-ñ?»)Ôì®Š¨8˜6ÇQŽvA?Ùf«ÞS)9#•äEª¼w•‰Ûë¶ƒZš£ä1Oe’gþ“^�b­õVc·´Ê9ñ/TÂt¬Lù˜¹ªéz¨hÁc.~9ØÑUx.Ð¹ijÀp±-ó_xË•å9æ
´M½Áÿ}ðeÔÆîm©Ð-Ó±Íü±�‚e1ƒZkT4T½0'‰“> 9*6 ûñâ§B÷æäñ¼¨šbQ`%Ò–íq¶®³‚´.à3-§lp»{í„Ê«ã‚Àå@ÚÆÙHäÑ6L¢8Î+˜6œ=âJ«ëw%Fg¤ëäÖRwk±Ö.±Ë‘¸@ûä¦3¹*ªfU¯áÒ¦õä[gRYÃ¢#TüÝ¦Buœ�žïU—|7»Š8ž¶­þÊüÄ$*}Ù-`/|‚�Qª{�M@ÛáÒð|ÃqŸ-Z­ƒ®•Ï€E9Ç%	ºƒ›ç~‚ŽâÓ´8ö?ö„4ŒÆþÌ`É;ûÝð¶zÉÙ]÷éù‰Z-ébåú&½û3»ó¹Áb„N�ÔŒÏ(S4³h‘íínW“ì·(¨,‚úþ´²˜+„§3%ÉOÂ+Aº2*s6xŽO‘ŒF­É±³MµæžD‘•Àí
ï¿€ðpßÿŠ7*P†­BÃÍÑºj£ÌB�¼ÃbÄbÑ”}°™p^ä»€ëÿ]<ívó«f˜oŒÍàôg `†Ð>Bf’�ÂK-©‡™¹&}þœFµ�fëúUs« ¥â)ˆ[½@ÊÂ€ÍÛû9Yž¿\Òø„Ùªñ¶¯žE4>$ÒÎ£”!ÎÍ7Xræ‚»e5®]›¦ô
÷ó•°³Õ¥»î'…aïZÒXüI!OÒöÖ  ÕEy’7ù’Ç6e‰IJ‡�zDÓ_½ ÁÆ¯=h�-ÇSQÐæiv¦mÈ®|äàg=¸…ªâÅ,Ž±ÌÒ�]¶H5àM4yeMúåŽyšPHL[› ;F~O¨¾Ô�LÇÂÖÅ,ídK6nÙ©ÈT¾4É"·$	¢<ºåß?'¬`Lc?®Õ¶ùN±ô‚�<Âã`‚êZ`6znh6èÎ9î<¨£]$÷,r¾OÍ§Õ	„Í’	2“z•§Nbª\p¥ZÜÁAƒ@¶ÿ}Pé°Ñ8ëÙ?­Òë‰�P¼ÙcCý$šfT,ÌÏh­`Ã4â2Õl™é¤-Ÿ¬½G1Ã®ÓÒ‘ÎÒïAãË*j1ã0i g…P[Tmùˆžwß&7S7´zT®„zÓïò¼:óˆ..¼6Ž»ãª¿º 0Ú÷’ßß½“âÄ9Çam:šÕ,uçê§Œá„UÎ-Nm¸Í¼sba*Ã &õØÅ Aoø‰H—§ðŠÙ«WÆw	pue�jµGþÎ[i¼Oš‹tò±ßÂ¡Eæèj‘­óÚõ49.-¢ö
-dMŠøD€�¡	¤»ì´u¸3Ì¶8äÉÈynUŸf™É¹'¶µÁÙà]™ÅÒÖ#Ã+¬	t.#ÓÌT{5T	
™mI'É,U8¾×›ÜœÅûa\èŠGÝ�Õp$¤€½lÄ[Ûî±]gPë1º‘!jAcË´[ƒƒy²,eÈ�±ùÿÈÅ­öÔŸ8ÛœŠ–j¥®o�4
™œ1·»ÌçíÀ7…Î›¡_d¹õ8I‹$­*Þ„8v�;£ÂžBÁ$‰crŸã1‡  ‚‰ešº·`~“þÂóåžzÄ~.ð–G"Ø“™nÙHeO]îß|6i¢Uè3g5n¯0™F3ÐL'�wlÎ Ù3¿h>øt�v†.€ãÌ%Ùþ£_o¤˜N½DLdýµD·Šü$·CŒ‘¼Ubä;ÇYe‘$ŠÌ
ÊyßÄU?GIE†SI$/È·F0YRvüâW  *º.JJ¦‚-rÊCIc¿hÍ+Í÷»˜ÙŸõV†qåÆf–‚rãpZÅ Õl^lÒÐrhª
KwEÃàù»Ú'°úñ¢H†¹‘v�·Š©h62á¢ã#°#ôcš”ÇFÎ0+*‰Ä‘j– ÷šïéƒÍ×œ0öÍµeþº+c<µtYHfå„w§†)µ‚páú8ß~sêŠ‡ÛR/)#>â9F½†-Ž—eÂí§ß|ðCï.€ÃYùr³9Òt;×	í hÓnp=Ä¬Ñ³2×¼|Ïr¤ a$‰J�y¯C#¶tØño’D¼7ý¸wñ¬—ËÕÙÝsøìzÌÈ¯£©å&‚æƒójÜÁ±¸x¿]â�I–×äŽ1ärbÀ�•%R|!Ÿ5^­ëKKJù&�QØT-á=#‹’êÝâ/¸WÑ3e•7ËoQ‚ÖÀ¢« �>‡/ïL¥›ÉÛ»å°¼£bÓ÷•ØØ‡ÎhMYyšÇÛFkt™MÊ\ƒóË(‡ß<öŸþÛ?€ ü GM   Nû¿6í­ç{ à¤ë ì~9o±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  $4<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="0" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2172" maxFusedRunOn="2172" detectionMode="exactProteinMatch"><Segment range="2143-2169" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6405" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5614-6405" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6406-6474" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="2" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3318-4112" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4858-5443" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2480-2908" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2922-3251" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2210-2434" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3102-3237" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4286-4407" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6475-6579" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-10" range="4504-4510" color="#ffffff" type="standard"/><Segment range="4511-4528" color="#ffffff" type="standard"/><Segment name="-35" range="4529-4534" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4549-4570" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4456-4472" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4480-4496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SERPINE1" directionality="1" translationMW="45061.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2172" maxFusedRunOn="2172"><Segment range="931-2136" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  §<Notes>
<UUID>da3fcf24-db51-4303-84dc-bc947c2938d4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-SERPINE1(human)-HA-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.9.17</Created>
<LastModified UTC="17:40:38">2025.9.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                              ©<StrandColors><TopStrand><ColorRange range="0..6642" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6642" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
